Based on a previous GBS approach, we designed bar code and adapter sets for the Ion Torrent platforms. To test new semiconductor sequencing platforms for GBS, we genotyped a barley recombinant inbred line (RIL) population. With quickly developing sequencing technologies, adapting current GBS methodologies to new platforms will leverage these advancements for future studies. Genotyping-by-sequencing (GBS), a low-cost, reduced representation sequencing method, is becoming a common approach for whole-genome marker profiling in many species. The rapid development of next-generation sequencing platforms has enabled the use of sequencing for routine genotyping across a range of genetics studies and breeding applications.
0 Comments
Leave a Reply. |